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Updated: Nov 29, 2025

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
Published on: November 26, 2018
Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators
Jere Weltner1,2,3, Ras Trokovic4
1Stem Cells and Metabolism Research Program, Biomedicum Stem Cell Center, University of Helsinki, Helsinki, Finland. jere.weltner@ki.se.
Abstract:
CRISPR-mediated gene activation (CRISPRa) can be used to target endogenous genes for activation. By targeting pluripotency-associated reprogramming factors, human fibroblasts can be reprogrammed into induced pluripotent stem cells (iPSCs). Here, we describe a method for the derivation of iPSCs from human fibroblasts using episomal plasmids encoding CRISPRa components. This chapter also provides procedure to assemble guide RNA cassettes and generation of multiplexed guide plasmids for readers who want to design their own guide RNAs.
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