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Protocol to Create Chronic Wounds in Diabetic Mice
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The dynamic wound microbiome.

Chunan Liu1,2, Alise J Ponsero1,2, David G Armstrong3

  • 1Department of Biosystems Engineering, University of Arizona, Tucson, AZ, USA.

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|November 24, 2020
PubMed
Summary
This summary is machine-generated.

Advanced sequencing technologies offer a more comprehensive understanding of diabetic foot ulcer (DFU) microbiology. Molecular diagnostics could enable personalized treatment, reducing amputations and healthcare costs.

Keywords:
Diabetic foot ulcerMetagenomicsNext-generation sequencingWound microbiome

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Area of Science:

  • Microbiology
  • Genomics
  • Medical Diagnostics

Background:

  • Diabetic foot ulcers (DFUs) are a major cause of diabetes-related amputations and hospitalizations, costing billions annually.
  • Current methods cannot predict which DFUs will heal versus those requiring amputation.
  • The growing prevalence of diabetes exacerbates the DFU problem.

Purpose of the Study:

  • To review the latest developments in DFU microbiology using sequencing technologies.
  • To discuss the potential and limitations of these advanced approaches for clinical application.
  • To explore the feasibility of molecular diagnostics for personalized DFU management.

Main Methods:

  • Review of current literature on sequencing technologies applied to DFU microbiome analysis.
  • Analysis of temporal patterns and system dynamics in DFU microbiome during wound progression.
  • Evaluation of functional annotations, including virulence and antibiotic resistance.

Main Results:

  • Sequencing technologies provide unbiased, high-resolution profiling of the DFU microbiome.
  • These methods reveal temporal microbiome shifts and system dynamics during wound healing and intervention.
  • Functional insights into virulence and antibiotic resistance are obtainable.

Conclusions:

  • Molecular tests during DFU visits can guide personalized treatment and antibiotic therapy.
  • Personalized management can decrease healthcare costs and improve patient outcomes.
  • This approach supports antibiotic stewardship and combats antimicrobial resistance.