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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
Published on: April 21, 2022
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Mass Spectrometric Analysis of Mitochondrial RNA Modifications
Yuma Ishigami1, Tsutomu Suzuki2, Takeo Suzuki3
1Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|November 24, 2020
Abstract:
Mitochondrial RNAs are modified posttranscriptionally. These modifications are required for proper functioning of RNA molecules, and thereby contribute to essential mitochondrial processes. Herein, we describe our latest mass spectrometry-based platform for analysis of posttranscriptional modifications of mitochondrial tRNAs, and measuring the in vitro activity of mitochondrial RNA-modifying enzymes.

