Simultaneous detection and speciation of mono- and di-valent copper ions with a dual-channel fluorescent nanoprobe

Redox cyclings between mono-/di-valent copper oxidation states occur in electron transfer reactions that underlie their biological functions. We report herein a dual channel fluorescent nanoprobe for the detection of mono- and di-valent copper ions. The probe, BSA-CDs@RBH/BCS, is designed by decorating carbon dots (CDs) on BSA encapsulated rhodamine hydrazide (RBH) and conjugating with bathocuproine disulfonate (BCS). Cu²⁺ catalyzes the hydrolysis of RBH, and the formed rhodamine B (RhB) shows emission at λ_ex/λ_em = 360/575 nm which ensures Cu²⁺ detection. BSA reduces Cu²⁺ to Cu⁺ and the BCS-Cu⁺ chelate shows emission at λ_ex/λ_em 360/450 nm which ensures Cu⁺ assay. Thus, the dual-channel fluorescence enables speciation of Cu²⁺ and Cu⁺.