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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Related Experiment Video

Updated: Nov 29, 2025

Synthesis and Characterization of Multi-Modal Phase-Change Porphyrin Droplets
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Developing a Microbubble-Based Contrast Agent for Synchrotron In-Line Phase Contrast Imaging.

Ngoc Ton, Una Goncin, Arash Panahifar

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    Summary
    This summary is machine-generated.

    Lipid-shelled microbubbles (MBs) larger than 4 μm show promise as contrast agents for X-ray phase contrast imaging (PCI). A concentration of 5 × 10^6 MBs/ml may be sufficient for visible phase contrast in vivo.

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    Area of Science:

    • Medical Imaging
    • Biophysics
    • Materials Science

    Background:

    • X-ray phase contrast imaging (PCI) offers enhanced soft tissue visualization compared to absorption-based methods.
    • Developing effective contrast agents is crucial for advancing PCI applications.

    Purpose of the Study:

    • To develop and assess ultrasound microbubbles (MBs) as contrast agents for X-ray in-line PCI.
    • To evaluate the impact of MB size, shell material, and concentration on phase contrast intensity.

    Main Methods:

    • Synthesized polydisperse perfluorobutane-core lipid-shelled MBs and polyvinyl-alcohol (PVA)-MBs.
    • Separated MBs into size groups (1-10 μm) and functionalized PVA-MBs with SPIONs.
    • Performed in-line PCI using filtered white beam micro-computed tomography and analyzed contrast intensity.

    Main Results:

    • Lipid-shelled MBs (6-10 μm and 4-6 μm) and SPION-coated PVA-MBs were detectable at various concentrations.
    • Increased positive contrast was observed with higher concentrations of lipid-shelled MBs.
    • A concentration of 5 × 10^6 MBs/ml showed potential for generating visible phase contrast.

    Conclusions:

    • Lipid-shelled MBs exceeding 4 μm are suitable candidates for X-ray PCI.
    • Optimal concentration for visible phase contrast appears to be around 5 × 10^6 MBs/ml.
    • Further development of MBs for PCI could lead to clinical translation.