Exploring diagnostic m6A regulators in endometriosis.

Area of Science:

• Reproductive biology
• Molecular biology
• Epigenetics

Background:

• Endometriosis is an estrogen-dependent inflammatory condition linked to infertility and pain.
• N6-methyladenosine (m6A) modification is crucial in gene expression regulation.
• The role of m6A regulators in endometriosis pathophysiology remains largely unexplored.

Purpose of the Study:

• To investigate the expression profiles of m6A regulators in endometriosis.
• To identify potential m6A-related diagnostic biomarkers for endometriosis.

Main Methods:

• Analysis of m6A regulator expression in normal, eutopic, and ectopic endometrial samples from public databases (GSE7305, GSE7307, GSE51981, E-MTAB-694).
• Profiling of 20 m6A regulators in 207 endometrial samples.
• Correlation analysis of m6A regulator expression with endometriosis severity and diagnostic potential.

Main Results:

• Most (19/20) m6A regulators were significantly downregulated in both eutopic and ectopic endometrium compared to normal endometrium.
• METTL3, YTHDF2, YTHDF3, HNRNPA2B1, HNRNPC, and FTO were commonly dysregulated.
• HNRNPA2B1 and HNRNPC expression correlated with endometriosis severity and showed diagnostic potential, potentially influencing immune cell infiltration.

Conclusions:

• Significant dysregulation of m6A regulators occurs in endometriosis.
• HNRNPA2B1 and HNRNPC are promising diagnostic biomarkers for endometriosis, potentially involved in immune response modulation.