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Related Experiment Video

Updated: Nov 28, 2025

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Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing.

Norbert Moldován1, Gábor Torma1, Gábor Gulyás1

  • 1Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.

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|November 25, 2020
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Summary

Long-read sequencing reveals complex bovine alphaherpesvirus 1 (BoHV-1) gene expression during infection. This study details viral mRNA, non-coding transcripts, and splice variants, uncovering intricate transcriptional overlaps.

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Area of Science:

  • Virology
  • Genomics
  • Transcriptomics

Background:

  • Bovine alphaherpesvirus 1 (BoHV-1) is a significant cattle pathogen.
  • Understanding BoHV-1's transcriptome is crucial for disease control.

Purpose of the Study:

  • To profile the dynamic lytic transcriptome of BoHV-1.
  • To annotate viral mRNAs, non-coding transcripts, and transcript isoforms.

Main Methods:

  • Utilized two long-read sequencing (LRS) techniques: Oxford Nanopore MinION and LoopSeq.
  • Employed multiple library preparation protocols for comprehensive analysis.
  • Annotated transcription start/end sites and splice variants.

Main Results:

  • Identified a large number of BoHV-1 transcript isoforms.
  • Demonstrated an extremely complex pattern of transcriptional overlaps.
  • Characterized viral mRNAs and non-coding transcripts.

Conclusions:

  • LRS methods provide a detailed view of the BoHV-1 lytic transcriptome.
  • The complex transcriptional landscape of BoHV-1 is now better understood.
  • Findings contribute to the study of viral gene expression.