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Related Experiment Video

Updated: Nov 28, 2025

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

Extracellular vesicles as potential tools for regenerative therapy.

Jose Antonio Rodriguez-Navarro¹, Jorge Pascual-Guerra¹, Silvia Sacristan¹

¹Instituto Ramón y Cajal de Investigaciones Sanitarias, IRYCIS Neurobiología-Investigación, Madrid, Spain.

Molecular & Cellular Oncology

|November 25, 2020

Summary

Young fibroblast-derived extracellular vesicles reduce lipid peroxidation in aging cells and tissues. These vesicles are rich in Glutathione-S-transferase Mu, providing potent antioxidant activity against cellular damage.

Keywords:

Small extracellular vesicles aging glutathione-S-transferase M lipid peroxidation senescence

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Area of Science:

Cellular senescence
Extracellular vesicles biology
Aging research

Background:

Cellular senescence is linked to aging and disease.
Lipid peroxidation is a marker of oxidative stress in aging.
Extracellular vesicles (EVs) mediate intercellular communication.

Purpose of the Study:

To investigate the effects of fibroblast-derived EVs on senescent cells.
To determine the antioxidant capacity of young fibroblast EVs.
To explore the therapeutic potential of EVs in mitigating age-related oxidative damage.

Main Methods:

Isolation and characterization of small extracellular vesicles from young human fibroblasts.
Treatment of senescent cells and old mice organs with these EVs.
Assessment of lipid peroxidation levels and antioxidant enzyme activity.

Main Results:

EVs from young fibroblasts significantly reduced lipid peroxidation in senescent cells.
Similar protective effects were observed in various organs of old mice.
Enrichment of Glutathione-S-transferase Mu (GST-Mu) with high lipid antioxidant activity was identified in the EVs.

Conclusions:

Small extracellular vesicles from young fibroblasts possess potent anti-lipid peroxidation properties.
GST-Mu in these EVs is a key factor in their antioxidant function.
Fibroblast-derived EVs show promise as a therapeutic strategy against age-related oxidative stress.