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Updated: Nov 28, 2025
10:11
Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
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Protein-recruiting synthetic molecules targeting the Golgi surface
Shunsuke Sawada1, Akinobu Nakamura, Tatsuyuki Yoshii
1Department of Nanopharmaceutical Sciences, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan. stsukiji@nitech.ac.jp.
Chemical Communications (Cambridge, England)
|November 25, 2020
Summary
Scientists developed new synthetic ligands that attach to the Golgi surface. These ligands control protein and lipid functions on the Golgi membrane, aiding cell biology research.
Area of Science:
- Cell Biology
- Chemical Biology
- Molecular Biology
Background:
- Organelle-specific small molecules are crucial for controlling cellular processes.
- Precisely localizing molecules within cells allows for targeted manipulation of function.
Purpose of the Study:
- To develop synthetic ligands that specifically target the Golgi apparatus.
- To demonstrate the utility of these ligands in controlling protein and lipid localization and function on the Golgi membrane.
Main Methods:
- Synthesis of novel small-molecule ligands.
- Cell-based assays to confirm Golgi localization.
- Experiments to assess recruitment of target proteins to the Golgi.
Main Results:
- Developed synthetic ligands with high specificity for the Golgi surface.
- Demonstrated rapid recruitment of cytoplasmic proteins to the Golgi using these ligands.
- Showcased the ability to manipulate Golgi membrane proteins and lipids.
Conclusions:
- These synthetic ligands provide a powerful chemical tool for spatiotemporal control of Golgi functions.
- The ligands enable new strategies for studying and manipulating cellular processes localized to the Golgi.
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