Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Material parameterization including damage for predicting diver lung underwater explosion (UNDEX) injury.

Journal of the mechanical behavior of biomedical materials·2026
Same author

Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.

Research square·2026
Same author

Endoluminal Catheter Pulsed Field Ablation for the Treatment of Atherosclerotic Vascular Disease.

Annals of biomedical engineering·2026
Same author

Microfluidics for cell therapy and manufacturing in oncology and regenerative medicine.

Lab on a chip·2026
Same author

High-Frequency Irreversible Electroporation Alters Proteomic Profiles and Tropism of Small Tumor-Derived Extracellular Vesicles to Promote Immune Cell Infiltration.

Cells·2025
Same author

Deep learning reveals how cells pull, buckle, and navigate fibrous environments.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Vertically Stacked Indium Gallium Zinc Oxide-Based Three-Dimensional Integrated Circuits.

ACS nano·2026
Same journal

Tunable Nanoparticle Thin-Film Reveals Distance Dependence of Auger-Mediated Radiation Enhancement in Diffuse Midline Glioma.

ACS nano·2026
Same journal

G-Quadruplex Network Engineering in Ionogels: Realizing Robust Biosensing Interfaces for Plant Electrophysiology.

ACS nano·2026
Same journal

Announcing the 2026 <i>ACS Nano</i> Lectureship and <i>ACS Nano</i> Impact Award Laureates.

ACS nano·2026
Same journal

Ultrafast Self-Assembly of Zeolitic Imidazolate Framework-8 Enables Antibody Orientation for Ultrasensitive Lateral Flow Immunoassays.

ACS nano·2026
Same journal

Interfacial Salt Engineering with Alkali and Ammonium Additives for Stable Pure-Blue Perovskite Light-Emitting Diodes and Micropatterned Displays.

ACS nano·2026
See all related articles

Related Experiment Video

Updated: Nov 28, 2025

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

13.6K

Single Cell Forces after Electroporation.

Philip M Graybill1, Aniket Jana1, Rakesh K Kapania2

  • 1Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia 24061, United States.

ACS Nano
|November 25, 2020
PubMed
Summary
This summary is machine-generated.

Electroporation alters cell contractility, causing initial force loss, a biphasic recovery with blebbing, and eventual restoration. Cell contractile force is a sensitive indicator of electroporation effects.

Keywords:
actincytoskeletonelectroporationforcesmechanobiologynanofiberspulsed electric fields

More Related Videos

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization
08:06

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization

Published on: September 27, 2024

820
Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells
10:12

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

Published on: September 6, 2024

528

Related Experiment Videos

Last Updated: Nov 28, 2025

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

13.6K
Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization
08:06

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization

Published on: September 27, 2024

820
Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells
10:12

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

Published on: September 6, 2024

528

Area of Science:

  • Cellular Biophysics
  • Mechanobiology
  • Electroporation Research

Background:

  • Electroporation uses high-voltage pulses to increase cell membrane permeability.
  • The impact of electroporation on cell contractility and cytoskeletal dynamics is not fully understood.
  • Understanding cell force changes post-electroporation is crucial for applications in genetic engineering and molecular medicine.

Purpose of the Study:

  • To investigate the dynamic changes in single-cell forces following electroporation.
  • To characterize the contractile signature and recovery stages of cells after electroporation.
  • To determine if cell contractility is a more sensitive metric than cell shape for assessing electroporation effects.

Main Methods:

  • Single-cell forces were measured using nanofibers as force sensors.
  • Glioblastoma cells were exposed to varying high-voltage pulses (500-1500 V) in parallel and perpendicular directions.
  • Cellular contractility, morphology, and viability were monitored over 1-3 hours post-electroporation.

Main Results:

  • Electroporation induced a three-stage recovery of cell contractility: initial force loss, a biphasic increase/decrease with blebbing, and final recovery.
  • Higher electric fields, especially perpendicular to cell orientation, significantly reduced cell viability.
  • Cellular contractile force proved more dynamic and sensitive to electroporation than cell shape changes.

Conclusions:

  • Cell contractility exhibits distinct dynamic changes post-electroporation, offering a sensitive measure of treatment effects.
  • The biphasic contractility stage is linked to actin disruption and blebbing, highlighting complex cellular responses.
  • These findings enhance understanding of electroporation's mechanobiological impact and have implications for various biomedical fields.