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Sandwich-Based Antibody Arrays for Protein Detection
Ying Qing Mao1, Brianne Petritis2, Ruo-Pan Huang2,3,4,5
1RayBiotech Life, Peachtree Corners, GA, USA. mike@raybiotech.com.
Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2020
Summary
This study introduces a rapid, 1-day method for processing samples using quantitative sandwich-based antibody arrays. This fluorescence-based technique allows for simultaneous detection of multiple proteins, offering a cost-effective alternative to traditional single-protein assays.
Area of Science:
- Biochemistry
- Immunotechnology
- Proteomics
Background:
- Sandwich-based antibody arrays allow for multiplexed protein detection.
- These arrays offer a time- and cost-effective alternative to single-plex assays.
- The "sandwich" technique involves a capture antibody and a detection antibody.
Purpose of the Study:
- To describe a streamlined 1-day procedure for sample processing.
- To utilize quantitative sandwich-based antibody arrays on a glass substrate.
- To enable fluorescence-based detection of multiple proteins.
Main Methods:
- Development of a 1-day protocol for sample processing.
- Application of quantitative sandwich-based antibody arrays.
- Utilizing fluorescence for protein detection on a glass substrate.
- Processing of biological samples like serum and cell lysates.
Main Results:
- Successful implementation of a 1-day procedure.
- Demonstration of multiplexed protein detection capabilities.
- Quantitative analysis of proteins in serum and cell lysates.
Conclusions:
- The described method provides an efficient and cost-effective approach for multiplexed protein analysis.
- This 1-day protocol facilitates rapid biomarker discovery and validation.
- The fluorescence-based antibody array platform is suitable for various biological samples.
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