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One-Step Antibody Arrays

Ying Qing Mao1, Lin-Hai Li2

  • 1RayBiotech Life, Peachtree Corners, GA, USA. mike@raybiotech.com.

Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2020
PubMed

The sandwich-based immunoassay is renowned for its specificity and sensitivity for protein detection, where the antigen is "sandwiched" by a pair of antigen epitope-specific antibodies. The capture antibody-antigen-detection antibody complex will be formed if all the components are present in a proper reaction system. A one-step rapid sandwich-based antibody array can be developed through fixing the capture antibody on a glass slide with a fluorescence-labelled detection antibody. In this chapter, we describe the process of a one-step mouse immunoglobulin isotyping array for use in hybridoma culture supernatants.

Keywords:
Antibody arrayFluorescence detectionOne-stepSandwich assay

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