Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Western Blotting01:15

Western Blotting

19.5K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
19.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic identification and validation of novel neuronal EV-based markers for Alzheimer's disease biomarker discovery.

bioRxiv : the preprint server for biology·2026
Same author

Coregulated metabolite networks associated with global protein crotonylation are central pathophysiological processes in prediabetes and diabetes.

medRxiv : the preprint server for health sciences·2026
Same author

Proteomic profiling of brain organoids and extracellular vesicles identifies early Alzheimer's disease biomarkers and drug response heterogeneity.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Automated Enrichment and Coextraction Pipeline for Efficient Analysis of Urinary Extracellular Vesicle Protein and RNA.

Journal of proteome research·2025
Same author

Host and bacterial urine proteomics might predict treatment outcomes for immunotherapy in advanced non-small cell lung cancer patients.

Frontiers in immunology·2025
Same author

Phosphoproteomics of Cerebrospinal Fluid-Derived Extracellular Vesicles Applied to Primary Central Nervous System Lymphoma.

Methods in molecular biology (Clifton, N.J.)·2025
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Nov 28, 2025

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

8.3K

Profiling Glycoproteins on Functionalized Reverse Phase Protein Array.

Ying Zhang1, Liyuan Zhang2, Anton Iliuk3

  • 1Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2020
PubMed
Summary
This summary is machine-generated.

A new polymer-based reverse phase glycoprotein array (polyGPA) platform enables sensitive detection of protein glycosylation. This method overcomes limitations of traditional assays, analyzing complex samples without specific antibody needs.

Keywords:
AntibodyDendrimerGlycosylationPost-translational modification (PTM)Reverse phase protein array

More Related Videos

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

16.0K
Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

1.2K

Related Experiment Videos

Last Updated: Nov 28, 2025

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

8.3K
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

16.0K
Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

1.2K

Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Reverse phase protein array (RPPA) is a high-throughput immunoassay for quantitative protein profiling.
  • Analyzing post-translationally modified (PTM) proteins, like glycosylation, using RPPA is challenging due to antibody specificity and low abundance.
  • Complex biological matrices in cell lysates and tissue extracts further complicate PTM detection.

Purpose of the Study:

  • To introduce a novel RPPA platform for sensitive detection of protein glycosylation.
  • To overcome the limitations of existing RPPA methods in analyzing PTMs.
  • To provide a strategy for analyzing glycosylation patterns in complex biological samples.

Main Methods:

  • Development of a polymer-based reverse phase glycoprotein array (polyGPA).
  • Utilizing a three-dimensionally functionalized RPPA platform.
  • Employing a method that does not require antibodies specific to glycosylation.

Main Results:

  • The polyGPA platform demonstrates a highly sensitive strategy for protein glycosylation analysis.
  • The method allows for parallel analysis of multiple complex biological samples.
  • Glycosylation patterns can be measured without the need for specific anti-glycosylation antibodies.

Conclusions:

  • The polymer-based reverse phase glycoprotein array (polyGPA) offers a significant advancement for PTM analysis.
  • This novel platform enhances the capability of RPPA in studying protein glycosylation.
  • PolyGPA provides a sensitive and versatile tool for glycomics research in complex samples.