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Related Concept Videos

Ion Exchange01:17

Ion Exchange

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Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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Cell Co-culture Patterning Using Aqueous Two-phase Systems
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Aqueous Two-Phase System (ATPS)-Based Polymersomes for Particle Isolation and Separation.

Hanjin Seo1, Changwoo Nam1,2, Eunseo Kim1

  • 1Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Korea.

ACS Applied Materials & Interfaces
|November 25, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method using aqueous two-phase systems (ATPS) in specialized droplets to efficiently separate extracellular vesicles (EVs). This technique overcomes limitations in isolating low-concentration biomolecules, enhancing purification strategies.

Keywords:
aqueous two-phase system (ATPS)isolationmicrofluidicspolymersomeseparation

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Area of Science:

  • Biochemistry
  • Biotechnology
  • Materials Science

Background:

  • Aqueous two-phase systems (ATPS) offer biocompatible separation of biomolecules.
  • Existing methods like ultracentrifugation and microfluidics struggle with low-concentration biomolecule isolation.
  • Extracellular vesicles (EVs) are crucial biomarkers requiring efficient separation techniques.

Purpose of the Study:

  • To develop an improved ATPS-based method for efficient separation and purification of biomolecules, particularly extracellular vesicles (EVs).
  • To address the limitations of current separation techniques in handling low concentrations of biomolecules.
  • To create stable and controllable ATPS droplets for enhanced biomolecule isolation.

Main Methods:

  • Utilized triple emulsion droplets with an oil phase to stabilize aqueous two-phase system (ATPS) droplets.
  • Engineered monodisperse ATPS-based polymersomes for enhanced stability and isolation.
  • Employed osmotic-pressure-induced rupture in a poly(ethylene glycol) (PEG) solution for selective EV separation within dextran (DEX)-rich droplets.

Main Results:

  • Successfully produced highly monodisperse and stable ATPS-based polymersomes.
  • Demonstrated effective isolation of model extracellular vesicle (EV) particles using the developed ATPS droplet system.
  • Achieved separation of EVs in a single dextran (DEX)-rich droplet through controlled polymersome rupture.

Conclusions:

  • The novel ATPS droplet strategy provides a robust and efficient method for isolating low-concentration biomolecules like EVs.
  • This approach enhances the stability and controllability of ATPS systems for biomolecule purification.
  • The technique offers a promising advancement for biomarker discovery and bioseparation applications.