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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
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RNA-seq identifies circulating miRNAs as potential biomarkers for plaque rupture in patients with ST-segment

Jiannan Li1, Yu Tan2, Zhaoxue Sheng1

  • 1Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

Genomics
|November 30, 2020
PubMed
Abstract

Insights

Three specific microRNAs (miR-744-3p, miR-324-3p, and miR-330-3p) can help differentiate plaque rupture from plaque erosion in acute coronary syndrome patients. This finding offers potential for improved diagnostic markers.

Area of Science:

  • Cardiovascular Medicine
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Acute coronary syndrome (ACS) involves two main pathological phenotypes: plaque rupture (PR) and plaque erosion (PE).
  • MicroRNAs (miRNAs) are implicated in the underlying mechanisms of PR and PE.
  • Understanding these mechanisms is crucial for accurate diagnosis and treatment of ACS.

Purpose of the Study:

  • To investigate the diagnostic utility of circulating miRNAs in differentiating between PR and PE.
  • To identify specific miRNA signatures associated with each plaque phenotype.
  • To assess the clinical applicability of miRNAs as diagnostic markers for ACS subtypes.

Main Methods:

  • Plasma samples were collected from patients with PR, PE, and healthy controls (HCs).
  • MicroRNA sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were employed for miRNA analysis.
  • Statistical analyses, including receiver operating characteristic (ROC) curve analysis, were performed to evaluate diagnostic performance.

Main Results:

  • Three miRNAs (miR-744-3p, miR-324-3p, and miR-330-3p) were significantly upregulated in the PR group compared to the PE group.
  • The combination of these three miRNAs demonstrated significant diagnostic utility in distinguishing PR from PE in both training and test sets, with areas under the ROC curve of 0.764 and 0.768, respectively.
  • The identified miRNA signature showed high sensitivity and specificity in differentiating the plaque phenotypes.

Conclusions:

  • Circulating miR-744-3p, miR-330-3p, and miR-324-3p are associated with plaque rupture (PR).
  • These miRNAs hold clinical utility as a diagnostic marker for differentiating plaque phenotypes in ST-elevation myocardial infarction (STEMI) patients.
  • This miRNA signature could aid in the precise diagnosis and management of acute coronary syndromes.
Keywords:
BiomarkerPlaque ruptureRNA-Seq

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