Luteimonas wenzhouensis Sp. Nov., A Chitinolytic Bacterium Isolated from a Landfill Soil

A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium with degradation ability of chitin, designated strain YD-1 ^T, was isolated from landfill soil sample collected in Wenzhou, Zhejiang province, China. The growth of strain YD-1 ^T occurred optimally in the tryptone soy broth (TSB) with 1.0% NaCl at pH 7.0-8.0, 30 °C. Ubiquinone-8 (Q-8) was the predominant quinone. The polar lipids of strain YD-1 ^T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, five glycolipids and four lipids. The major fatty acids were iso-C_15:0 (30.7%), iso-C_17:1ω9c (23.2%), iso-C_11:0 (18.9%), iso-C_11:0 3-OH (6.8%) and iso-C_17:0 (5.9%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YD-1 ^T was affiliated to the genus Luteimonas with the highest similarity to Luteimonas marina KCTC 12327 ^T (97.3%), followed by Luteimonas aquatica DSM 22088 ^T (96.5%) and Luteimonas composti CCUG 53595 ^T (96.4%). The genomic DNA G + C content of strain YD-1 ^T was 71.8 mol%. Average nucleotide identity (ANI) and the digital DNA-DNA hybridizations (dDDH) for draft genomes between strain YD-1 ^T and Luteimonas marina KCTC 12327 ^T were 82.7% and 26.1%, respectively. On the basis of genotypic, phenotypic and chemotaxonomic data, strain YD-1 ^T is considered to represent a novel species to degrade chitin in the genus Luteimonas, for which the name Luteimonas wenzhouensis sp. nov. is proposed, with YD-1 ^T (= KCTC 72425 ^T = CCTCC AB 2019153 ^T) as the type strain.