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Updated: Nov 28, 2025
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Published on: April 20, 2018
Establishment of an in vitro model using the rat alveolar macrophage cell line NR8383
Jiawei Wang1, Xuekai Wang1, Yu Sui2
1Department of Oncology, Guanganmen Hospital of Chinese Academy of Chinese Medicine Science, Beijing 100053, China.
Objective:
To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages (NR8383).
Methods:
Using a medical electronic linear accelerator, cells were irradiated with either 0 Gy or 6 Gy X-rays. At 6, 12, 24, 30 and 48 h, the DNA damage index (8-OHdG) and lipid damage index (MDA) were measured in the two groups. We also determined the levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and transforming growth factor-β (TGF-β).
Results:
The levels of 8-OHdG and MDA in the 6 Gy irradiation group were higher than those in the 0 Gy group at 6, 12, 24, 30 and 48 h after irradiation. The levels reached the highest value -6 h after irradiation, and then gradually decreased. The levels of the inflammatory factors TNF-α, TGF-β and IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6, 12, 24, 30 and 48 h after irradiation.
Conclusion:
Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury. The levels of 8-OHdG, MDA, TNF-α, TGF-β and IL-6 can be used as effective evaluation indicators.
