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[Interactions between macrophages and anti-infective agents].

J L Vildé1, R Rajagopalan, J J Pocidalo

  • 1INSERM U13, Hôpital Claude-Bernard, Paris.

Pathologie-Biologie
|December 1, 1987
PubMed
Summary

Antimicrobial drugs can improve macrophage bacteria uptake at low doses. Macrolides and fluoroquinolones show good intracellular activity against bacteria within macrophages, unlike beta-lactams.

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Area of Science:

  • Immunology
  • Microbiology
  • Pharmacology

Context:

  • Macrophage function is crucial for host defense against bacterial infections.
  • The direct impact of antimicrobial agents on macrophage functions, particularly intracellular activity, requires further elucidation.
  • Previous studies show varied effects of antimicrobials on macrophage phagocytosis at subinhibitory concentrations.

Purpose:

  • To investigate the direct effects of various antimicrobial agents on macrophage functions.
  • To assess the intracellular activity of different antimicrobial classes against Legionella pneumophila within human monocyte-derived macrophages.
  • To determine the relationship between antimicrobial concentration, minimum inhibitory concentration (MIC), and intracellular efficacy.

Summary:

  • Antimicrobial agents exhibit varied effects on macrophage functions, including phagocytosis enhancement at subinhibitory concentrations.
  • In a human monocyte-derived macrophage model, erythromycin, rifampicin, and fluoroquinolones effectively inhibited intracellular Legionella pneumophila growth at sub-MIC levels.
  • Doxycycline and cotrimoxazole showed inhibitory effects near their MICs, while cefoxitin demonstrated poor intracellular activity despite a low MIC.

Impact:

  • This study highlights the differential intracellular efficacy of antimicrobial agents, confirming strong activity for macrolides and fluoroquinolones.
  • Findings suggest that macrolides and fluoroquinolones are promising agents for treating intracellular bacterial infections involving macrophages.
  • The results underscore the limited intracellular effectiveness of beta-lactams, guiding therapeutic choices for infections where macrophages play a key role.

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