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Paper-disc method for Campylobacter hippurate-hydrolysis test. A note.

I H Kotsis¹, M Adám

¹National Institute of Hygiene, Budapest, Hungary.

Acta Microbiologica Hungarica

|January 1, 1987

Summary

A new hippurate hydrolysis test using paper discs accurately identifies Campylobacter jejuni. This method confirmed results from a standard test, finding C. jejuni in 59.8% of human and 41.7% of poultry samples.

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Area of Science:

Microbiology
Clinical Diagnostics
Food Safety

Background:

Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide.
Accurate and rapid identification of Campylobacter species is crucial for public health and food safety.
Existing methods for Campylobacter identification can be time-consuming or require specialized equipment.

Purpose of the Study:

To evaluate a novel hippurate hydrolysis test for the rapid identification of Campylobacter strains.
To compare the efficacy of this new method with a modified rapid method by Skirrow and Benjamin.

Main Methods:

Paper discs impregnated with sodium hippurate were used as the substrate.
The test involved incubating bacterial suspensions with the discs, followed by the addition of ninhydrin reagent.
169 Campylobacter strains from human and poultry sources were tested.

Main Results:

The hippurate hydrolysis test demonstrated identical results to the modified Skirrow and Benjamin method for all 169 strains tested.
Campylobacter jejuni was identified in 59.8% of human fecal isolates and 41.7% of poultry isolates.

Conclusions:

The sodium hippurate paper disc test is a reliable and effective method for identifying Campylobacter jejuni.
This method offers a rapid and accessible diagnostic tool for Campylobacter detection in clinical and food safety settings.

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