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[Phenotypic and genotypic properties of 4 virulence plasmids of Salmonella typhimurium]

P Pohl1, P Lintermans, F Bex

  • 1Institut National de Recherches Vétérinaires, Université libre de Bruxelles.

Annales De L'Institut Pasteur. Microbiology
|September 1, 1987
PubMed

Insights

Four virulence-associated plasmids confer the fi+ character but do not encode adhesins, aerobactin, or colicin synthesis. These plasmids contain specific DNA sequences homologous to FIIA and FIB replicons, aiding in their characterization.

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Context:

  • Virulence-associated plasmids play a crucial role in bacterial pathogenicity.
  • Understanding plasmid-mediated traits is essential for combating bacterial infections.

Purpose:

  • To characterize the functional and genetic properties of specific virulence-associated plasmids (pLT2, pIP1350, pIP1360, pIP1370).
  • To determine if these plasmids confer traits such as fi+ character, adhesin synthesis, aerobactin, colicin production, or metal resistance.

Summary:

  • Plasmids pLT2, pIP1350, pIP1360, and pIP1370 were found to code for the fi+ character.
  • These plasmids did not exhibit genes for classical adhesins, aerobactin, colicins, or resistance to Hg, Te, As, Cd, or flavomycin.
  • Sequence analysis revealed homology to incFIIA and incFIB replicons in pLT2, pIP1350, and pIP1370, while pIP1360 showed homology only to incFIIA.

Impact:

  • Provides detailed genetic characterization of key virulence plasmids.
  • Contributes to understanding the genetic basis of bacterial virulence and plasmid evolution.
  • Informs future research on plasmid-mediated pathogenesis and potential therapeutic targets.

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