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Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitors
Emiko Okubo-Kurihara1, Wen-Dee Ong2, Yukio Kurihara1
1RIKEN, Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2020
Summary
This study identifies a novel inhibitor of cryptochromes, blue-light photoreceptors crucial for plant development. Phenotypic screening in Arabidopsis thaliana seedlings accelerates the discovery of compounds impacting photomorphogenesis.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Plants exhibit photomorphogenesis, controlling development through light responses after germination.
- Cryptochromes are key blue-light photoreceptors regulating vital processes like de-etiolation and flowering time.
Purpose of the Study:
- To develop a phenotypic screening system for blue-light responses in Arabidopsis thaliana.
- To identify compounds that modulate cryptochrome activity.
Main Methods:
- Utilized Arabidopsis thaliana seedlings for phenotypic screening.
- Focused on blue-light-dependent developmental processes.
- Employed a chemical screening system to isolate active compounds.
Main Results:
- Successfully established a phenotypic screening system for blue-light responses.
- Identified a compound that inhibits cryptochrome function.
Conclusions:
- The developed screening system effectively identifies cryptochrome inhibitors.
- This approach facilitates rapid discovery of compounds affecting plant photomorphogenesis.
