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Updated: Nov 27, 2025
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
Extensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid
Anelise Stella Ballaben1, Renata Galetti1, Leonardo Neves Andrade1
1Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Abstract:
IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-β-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. putida group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP-16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed blaIMP-16 as a gene cassette on class 1 integron, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, blaIMP-16, blaOXA-2, sul1) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Galleria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir.
Insights
Multidrug-resistant Pseudomonas monteilii carrying the IMP-16 carbapenemase gene emerged. This study sequenced an extensively drug-resistant strain, revealing its genetic makeup and potential as a reservoir for resistance genes.
Area of Science:
- Microbiology
- Genomics
- Antimicrobial Resistance
Background:
- Metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is globally detected.
- Pseudomonas monteilii, previously low virulence, now shows multidrug and carbapenem resistance.
- The emergence of resistant strains necessitates understanding their genetic basis.
Purpose of the Study:
- To report the draft genome sequence of an extensively drug-resistant IMP-16-producing P. monteilii.
- To characterize the genetic elements, including integrons and resistance genes, within this strain.
- To investigate the virulence potential of this multidrug-resistant isolate.
Main Methods:
- Whole-genome sequencing of P. monteilii 597/14.
- Identification and characterization of resistance genes and integrons.
- Virulence assessment using the Galleria mellonella infection model.
Main Results:
- Draft genome sequence of extensively drug-resistant P. monteilii 597/14 obtained.
- blaIMP-16 identified as a gene cassette within a novel class 1 integron, In1738.
- The strain harbored seven resistance genes and multiple virulence determinants but showed low virulence in G. mellonella.
Conclusions:
- P. monteilii can act as a reservoir for clinically significant resistance genes, including carbapenemases.
- The genetic characterization of this strain provides insights into the spread of antimicrobial resistance.
- Further surveillance of P. monteilii is crucial for monitoring emerging resistance threats.
