Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial sensitivity distributions for biocides and metals.

FEMS microbiology ecology·2026
Same author

Probiotic and Technological Potential of Enterococcus faecium EM03, Isolated From Brazilian Semi-hard Cheese.

Probiotics and antimicrobial proteins·2026
Same author

Genomic characterization of high-risk ST340 and ST437 Klebsiella pneumoniae co-harboring bla<sub>NDM-1</sub> and bla<sub>KPC-2</sub> from wastewater.

International journal of antimicrobial agents·2026
Same author

Antibiotic-Induced Pulmonary Fibrosis: National Database Analysis.

Biomedicines·2026
Same author

Proposal for screening cut-off values and use of tolerance disk test (TDtest) for detection of tolerance/persistence to ceftazidime-avibactam in Klebsiella pneumoniae.

Diagnostic microbiology and infectious disease·2026
Same author

Enhanced activity and operational stability of recombinant acetylxylan esterase from Aspergillus nidulans immobilized on amino-functionalized magnetic nanoparticles.

Enzyme and microbial technology·2026

Related Experiment Video

Updated: Jul 13, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.1K

Applying 3D cultures and high-throughput technologies to study host-pathogen interactions.

Elaine Cristina Pereira De Martinis1, Virgínia Farias Alves2, Marita Gimenez Pereira1

  • 1Ribeirão Preto School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

Frontiers in Immunology
|March 7, 2025
PubMed
Summary

Three-dimensional (3D) cell cultures offer advanced models for studying host-microbe interactions. Integrating these systems with molecular methods can reveal complex relationships within the human microbiome.

Keywords:
high-throughput sequencingmicrobial model communitiesorgan-on-a-chiporganoidsrotating wall vessel (RWV)transposon sequencing (TnSeq)tridimensional cell culture

More Related Videos

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

9.7K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.0K

Related Experiment Videos

Last Updated: Jul 13, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.1K
High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

9.7K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.0K

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Human microbiome research has advanced significantly due to innovations in cell culturing and DNA sequencing.
  • Three-dimensional (3D) cell culture models mimic in vivo conditions, offering reliable and reproducible in vitro systems.
  • These models are crucial for cell biology, cancer research, and understanding host-microbe interactions.

Purpose of the Study:

  • To provide an overview of 3D culture techniques for studying host-pathogen interactions.
  • To explore the integration of 3D culture systems with high-throughput molecular methods.
  • To discuss the potential of multi-species models for microbiome research.

Main Methods:

  • Review of established and emerging 3D cell culture techniques (e.g., spheroids, organoids).
  • Integration strategies with high-throughput molecular techniques like genomics, transcriptomics, and proteomics.
  • Development and application of multi-species co-culture systems.

Main Results:

  • 3D cell cultures effectively replicate in vivo host microenvironments for studying microbial pathogenesis.
  • Combined approaches enable detailed analysis of host responses and microbial dynamics.
  • Multi-species models facilitate the study of complex interactions involving commensal, beneficial, and pathogenic microbes.

Conclusions:

  • 3D cell culture systems are powerful tools for advancing human microbiome research.
  • Integration with molecular methods enhances the depth of understanding host-microbe interactions.
  • Future research directions include sophisticated multi-species models for comprehensive microbiome analysis.