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Related Experiment Video

Updated: Nov 27, 2025

Obtaining Eggs from Xenopus laevis Females
04:47

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

15.0K

Obtaining Xenopus laevis Eggs.

Nikko-Ideen Shaidani1, Sean McNamara1, Marcin Wlizla1

  • 1National Xenopus Resource, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA.

Cold Spring Harbor Protocols
|December 4, 2020
PubMed
Summary

Induce ovulation in Xenopus laevis frogs using hormone injections for year-round egg production. This method allows for large-scale embryo generation and egg extract preparation, simplifying research applications.

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Area of Science:

  • Reproductive biology
  • Amphibian research
  • Developmental biology

Background:

  • Ovulation in Xenopus laevis was historically induced using mammalian gonadotropins.
  • This hormonal induction allows for year-round egg production, independent of natural reproductive cycles.
  • The process enables the generation of thousands of eggs for fertilization or egg extract production.

Purpose of the Study:

  • To provide a simplified and clear protocol for inducing ovulation in Xenopus laevis.
  • To enable researchers, particularly those new to Xenopus laevis, to reliably collect eggs.
  • To standardize egg collection methods for consistent experimental outcomes.

Main Methods:

  • Injection of mammalian gonadotropic hormones into the dorsal lymph sac of female Xenopus laevis.

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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

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Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
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Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

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Related Experiment Videos

Last Updated: Nov 27, 2025

Obtaining Eggs from Xenopus laevis Females
04:47

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Published on: June 6, 2021

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Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
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Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

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  • Gentle manual pressure applied to stimulate egg laying post-hormone administration.
  • Focus on a specific, robust protocol used at the National Xenopus Resource.

    • Main Results:

    • Successful induction of ovulation and subsequent egg collection in Xenopus laevis.
    • Consistent egg production independent of natural breeding seasons.
    • Generation of a large number of eggs suitable for various research applications.

    Conclusions:

    • The described protocol offers a straightforward and reliable method for Xenopus laevis egg induction and collection.
    • This technique is valuable for researchers requiring a consistent supply of eggs for developmental studies or other applications.
    • Standardizing this protocol facilitates broader accessibility and application in amphibian research.