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Obtaining Eggs from Xenopus laevis Females

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

Obtaining Xenopus laevis Eggs.

Nikko-Ideen Shaidani¹, Sean McNamara¹, Marcin Wlizla¹

¹National Xenopus Resource, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA.

Cold Spring Harbor Protocols

|December 4, 2020

Summary

This summary is machine-generated.

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Induce ovulation in Xenopus laevis frogs using hormone injections for year-round egg production. This method allows for large-scale embryo generation and egg extract preparation, simplifying research applications.

Area of Science:

Reproductive biology
Amphibian research
Developmental biology

Background:

Ovulation in Xenopus laevis was historically induced using mammalian gonadotropins.
This hormonal induction allows for year-round egg production, independent of natural reproductive cycles.
The process enables the generation of thousands of eggs for fertilization or egg extract production.

Purpose of the Study:

To provide a simplified and clear protocol for inducing ovulation in Xenopus laevis.
To enable researchers, particularly those new to Xenopus laevis, to reliably collect eggs.
To standardize egg collection methods for consistent experimental outcomes.

Main Methods:

Injection of mammalian gonadotropic hormones into the dorsal lymph sac of female Xenopus laevis.

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Last Updated: Nov 27, 2025

Obtaining Eggs from Xenopus laevis Females

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

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Published on: June 6, 2021

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

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Published on: May 23, 2010

Gentle manual pressure applied to stimulate egg laying post-hormone administration.

Focus on a specific, robust protocol used at the National Xenopus Resource.

Main Results:

Successful induction of ovulation and subsequent egg collection in Xenopus laevis.
Consistent egg production independent of natural breeding seasons.
Generation of a large number of eggs suitable for various research applications.

Conclusions:

The described protocol offers a straightforward and reliable method for Xenopus laevis egg induction and collection.
This technique is valuable for researchers requiring a consistent supply of eggs for developmental studies or other applications.
Standardizing this protocol facilitates broader accessibility and application in amphibian research.