Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

370
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
370
Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

566
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
566
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

306
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
306
Yeast Signaling01:28

Yeast Signaling

16.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
16.7K
Peptidoglycan Synthesis01:28

Peptidoglycan Synthesis

1.3K
Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan...
1.3K
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

575
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
575

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The emerging dialogue between synthetic genomics and clinical research.

Clinical and translational medicine·2026
Same author

A risk-based post ablation follow-up strategy for hepatocellular carcinoma.

JHEP reports : innovation in hepatology·2026
Same author

Yeast nuclei-mediated precise delivery of synthetic megabase-scale human DNA into mammalian embryos.

Nature protocols·2026
Same author

RHEBL1 expression is activated by the Oct4-Sox2 complex in oral squamous cell carcinoma.

Functional & integrative genomics·2026
Same author

Correction: The PurR family transcriptional regulator promotes butenyl-spinosyn production in Saccharopolyspora pogona.

Applied microbiology and biotechnology·2026
Same author

High-Coordination Single-Atom Nanozyme-Based Colorimetric-Photothermal Sensing Platform for Evaluation of Total Antioxidant Capacity.

Analytical chemistry·2026

Related Experiment Video

Updated: Nov 27, 2025

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.7K

Pathway engineering in yeast for synthesizing the complex polyketide bikaverin.

Meng Zhao1,2,3, Yu Zhao3, Mingdong Yao1,2

  • 1Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, PR China.

Nature Communications
|December 4, 2020
PubMed
Summary

Researchers engineered the bikaverin biosynthetic pathway in yeast, increasing production by 273-fold. This demonstrates yeast

More Related Videos

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.5K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.2K

Related Experiment Videos

Last Updated: Nov 27, 2025

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.7K
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.5K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.2K

Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Natural product biosynthesis

Background:

  • Fungal polyketides possess diverse bioactivities and structural complexity.
  • Engineering microbial hosts for polyketide production is therapeutically significant.

Purpose of the Study:

  • To establish and engineer the bikaverin biosynthetic pathway in Saccharomyces cerevisiae.
  • To enhance bikaverin production for potential therapeutic applications.

Main Methods:

  • Utilized a green fluorescent protein (GFP) mapping strategy to identify pathway bottlenecks.
  • Employed a promoter exchange strategy to upregulate key enzymes.
  • Implemented an enzyme-fusion strategy for improved intermediate channeling.

Main Results:

  • Identified low expression of Bik1 (polyketide synthase) as a bottleneck.
  • Increased bikaverin titer by 273-fold, reaching 202.75 mg/L via flask fermentation.
  • Successfully established and optimized a complex fungal polyketide pathway in yeast.

Conclusions:

  • Saccharomyces cerevisiae is a viable host for engineering complex fungal polyketide biosynthesis.
  • The developed strategies significantly enhance bikaverin production.
  • This work highlights the potential for yeast in natural product synthesis and large-scale fermentation.