Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysosome-derived methylated arginine is a signalling metabolite controlling the lipidome.

Nature cell biology·2026
Same author

Functional asymmetry and essential structural roles of PDE6α and PDE6β subunits in rod-photoreceptor integrity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

DICER-driven regulation of lipid metabolism and macrophage-adipocyte crosstalk in partial lipodystrophy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Microproteins at the Frontiers of Biology and Medicine.

Annual review of cell and developmental biology·2026
Same author

Regional and Systemic Metabolic Remodeling Promotes Longevity by Bioengineered Yeast-Derived Lipids.

bioRxiv : the preprint server for biology·2026
Same author

Label free multimodal optical imaging of metabolic heterogeneity in aging by integrating SRS, MPF, FLIM, and SHG.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 27, 2025

Electroretinogram Analysis of the Visual Response in Zebrafish Larvae
09:44

Electroretinogram Analysis of the Visual Response in Zebrafish Larvae

Published on: March 16, 2015

15.9K

Elovl2 Is Required for Robust Visual Function in Zebrafish.

Manish Dasyani1, Fangyuan Gao2, Qianlan Xu1,2

  • 1Viterbi Family Department of Ophthalmology, Shiley Eye Institute, School of Medicine, University of California San Diego, La Jolla, CA 92093, USA.

Cells
|December 5, 2020
PubMed
Summary
This summary is machine-generated.

Inactivating ELOVL2 in zebrafish embryos altered lipid composition, particularly docosahexaenoic acid (DHA) levels. This genetic change also affected visual behavior, highlighting the role of long-chain polyunsaturated fatty acids in retinal health.

Keywords:
ELOVL2 (Elongation of Very Long Chain Fatty Acids-Like 2)Muller gliapolyunsaturated fatty acidsvision

More Related Videos

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
09:00

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Published on: February 11, 2022

4.2K
The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish
04:56

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish

Published on: October 9, 2013

20.8K

Related Experiment Videos

Last Updated: Nov 27, 2025

Electroretinogram Analysis of the Visual Response in Zebrafish Larvae
09:44

Electroretinogram Analysis of the Visual Response in Zebrafish Larvae

Published on: March 16, 2015

15.9K
Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
09:00

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Published on: February 11, 2022

4.2K
The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish
04:56

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish

Published on: October 9, 2013

20.8K

Area of Science:

  • Ophthalmology
  • Neuroscience
  • Molecular Biology

Background:

  • Polyunsaturated fatty acids (PUFAs) are vital for retinal function, influencing membrane stability and cell signaling.
  • Elongation of Very Long Chain Fatty Acids-Like 2 (ELOVL2) is an enzyme crucial for synthesizing long-chain PUFAs (LC-PUFAs) and has been linked to retinal aging.

Purpose of the Study:

  • To investigate the expression and functional role of the ELOVL2 gene in zebrafish retinal development.
  • To understand the impact of ELOVL2 inactivation on lipid composition and visual function in zebrafish.

Main Methods:

  • Whole mount in situ hybridization was used to determine elovl2 expression patterns in zebrafish retinas.
  • Lipidomics analysis was performed on elovl2 crispants to assess changes in lipid profiles.
  • Histological analysis examined retinal structure, and visual motor reflex (VMR-OFF) tests evaluated visual behavior.

Main Results:

  • elovl2 was found to be expressed in Muller glia in both embryonic and adult zebrafish retinas.
  • Inactivation of elovl2 led to significant alterations in lipid composition, notably affecting docosahexaenoic acid (DHA)-containing lipids.
  • Zebrafish lacking elovl2 exhibited increased retinal thickness and altered visual motor reflex responses.

Conclusions:

  • ELOVL2 plays a significant role in regulating lipid metabolism, specifically DHA synthesis, within the developing zebrafish retina.
  • The findings underscore the importance of LC-PUFAs, regulated by ELOVL2, for maintaining healthy retinal structure and visual function.