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Related Experiment Video

Updated: Nov 27, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Temperature-Induced uncoupling of cell cycle regulators.

Hanieh Falahati¹, Woonyung Hur², Stefano Di Talia²

¹Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08544, USA.

Developmental Biology

|December 5, 2020

Summary

Cell cycle progression in early Drosophila embryos is reproducible across temperatures. However, Cdk1 activation specifically drives prometaphase entry, revealing distinct regulatory mechanisms for mitosis.

Keywords:

Arrhenius dependence Cell cycle regulation Regulation of biological networks

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

Early embryonic development involves complex, reproducible morphological changes.
These developmental processes often exhibit robustness against environmental variations.

Purpose of the Study:

Investigate the relationship between reproducibility and robustness in cell cycle progression.
Examine how temperature affects cell cycle timing and regulation in early Drosophila embryos.

Main Methods:

Observed cell cycle progression in Drosophila embryos across a temperature range (5-35°C).
Utilized an in vivo biosensor to quantify the activity ratio of Cdk1 and PP1.
Pharmacologically inhibited Cdk1 activity to assess its role in mitosis.

Main Results:

Cell cycle step subdivision remained conserved across temperatures, but step durations varied.
Prometaphase entry was delayed at lower temperatures, suggesting unique regulatory mechanisms.
Cdk1 activation drives prometaphase entry but is not essential for earlier events like chromosome condensation.

Conclusions:

Different kinases act as rate-limiting factors for distinct mitotic stages.
Robust inter-regulation between kinases is crucial for rapid and ordered mitosis.
Temperature influences cell cycle dynamics, highlighting conserved yet adaptable developmental processes.