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Related Experiment Video

Updated: Nov 27, 2025

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

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Engineering approaches for effective therapeutic applications based on extracellular vesicles.

Seunglee Kwon¹, Sol Shin², Minjae Do³

¹School of Chemical Engineering, College of Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|December 5, 2020

Summary

Extracellular vesicles (EVs) are key for cell communication and show promise as biotherapeutics. This review explores engineering strategies to enhance therapeutic EVs and target disease-causing EVs for better disease intervention.

Keywords:

Biogenesis inhibition Drug loading Extracellular vesicles Preconditioning Surface modification Therapeutic applications

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Evaluation of the Storage Stability of Extracellular Vesicles

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Published on: May 22, 2019

Area of Science:

Biotechnology
Cell Biology
Regenerative Medicine

Background:

Extracellular vesicles (EVs) mediate intercellular communication in physiological and disease processes.
EVs possess regenerative and immunomodulatory properties, making them potential biotherapeutics.
Pathological EVs contribute to disease and are targets for therapeutic inhibition.

Purpose of the Study:

To provide an updated overview of engineering approaches for utilizing EVs in disease intervention.
To highlight methods for enhancing therapeutic EVs and controlling pathological EVs.
To discuss the use of EVs as drug delivery vehicles.

Main Methods:

Review of current literature on EV engineering for therapeutic applications.
Analysis of preconditioning methods for therapeutic EVs.
Examination of drug loading and targeting strategies for carrier EVs.
Evaluation of strategies for controlling the activity of pathological EVs.

Main Results:

EVs can be engineered for enhanced therapeutic efficacy and targeted drug delivery.
Preconditioning improves the regenerative and immunomodulatory potential of therapeutic EVs.
Strategies exist to inhibit the bioactivity of pathological EVs.
EV engineering offers versatile approaches for disease intervention.

Conclusions:

Engineering EVs is crucial for maximizing their therapeutic potential and minimizing pathological effects.
Advanced EV engineering techniques are vital for developing novel biotherapeutics and drug delivery systems.
This review provides a comprehensive guide to EV engineering for diverse clinical applications.