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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Related Experiment Video

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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Using Simple-Structured Split Aptamer for Gold Nanoparticle-based Colorimetric Detection of Estradiol.

Chia-Chen Chang1,2, Chung-Yu Yeh3

  • 1Department of Medical Biotechnology and Laboratory Sciences College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan. chang@cgu.edu.tw.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|December 7, 2020
PubMed
Summary

A new colorimetric biosensor uses split aptamers for sensitive estradiol detection. This method offers a faster, more affordable alternative for detecting this important hormone in clinical settings.

Keywords:
Split aptamercolorimetric assayestradiol detectiongold nanoparticlelocalized surface plasmon resonance

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Estradiol detection is crucial, with increasing demand for sensitive methods.
  • Existing gold nanoparticle aptasensors face sensitivity limitations due to aptamer secondary structures.
  • Split aptamer strategies offer a potential solution to enhance biosensor performance.

Purpose of the Study:

  • To develop a sensitive, label-free colorimetric biosensor for estradiol detection.
  • To investigate the impact of split aptamer secondary structure free energy on sensor sensitivity.
  • To evaluate the potential of the developed biosensor for clinical applications.

Main Methods:

  • Utilized gold nanoparticles for colorimetric detection.
  • Employed an estradiol-specific split aptamer system.
  • Optimized split aptamer selection based on secondary structure free energy (ΔG).
  • Established a standard calibration curve for estradiol quantification.

Main Results:

  • A superior sensor response was achieved using split aptamers with high free energy (ΔG > -3 kcal/mol).
  • The optimized biosensor demonstrated a detection limit of 6.7 nM for estradiol.
  • A wide linear range of 6.7 nM - 66.7 μM was established.
  • The assay provided results within 50 minutes, highlighting its speed and ease of use.

Conclusions:

  • The developed split aptamer-based colorimetric biosensor offers high sensitivity and efficiency for estradiol detection.
  • This method is cost-effective, rapid, and suitable for clinical applications.
  • The approach shows promise for detecting other small molecular targets.