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Plasmonic-based impedance microspectroscopy of optically heterogeneous samples.

Sidahmed A Abayzeed¹

¹Optics and Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham, NG7 2RD, UK.

Biomedical Optics Express

|December 7, 2020

Summary

This study introduces a new impedance microscopy technique for high-resolution imaging of electrical properties. The method corrects for optical variations, enabling precise measurements of cell and biomolecule impedance.

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Area of Science:

Biophysics
Optical Microscopy
Electrical Impedance Spectroscopy

Background:

Surface plasmon resonance (SPR) sensors can measure surface charge density, enabling impedance computation for adhered cells.
Quantitative impedance imaging is challenged by optical heterogeneity at the cell-sensor interface.

Purpose of the Study:

To develop a robust impedance microscopy technique for high-resolution imaging of electrical properties.
To address challenges in quantitative impedance imaging caused by optical heterogeneity.

Main Methods:

A novel quantitative time-resolved resonance angle tracking method was developed.
The technique corrects for optical properties affecting impedance measurements.
Impedance microspectroscopy was performed on bovine serum albumin (BSA) patterns.

Main Results:

The developed method enables reliable quantitative impedance imaging.
Capacitance measurements with submicroscopic resolution were achieved.
The technique successfully corrected for optical heterogeneity.

Conclusions:

The presented impedance microspectroscopy method offers new avenues for studying single-cell and biomolecule electrical properties.
This technique has promising biophysical applications, including the study of bio-electrical currents.