Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

582
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
582

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anterior cingulate cortex neuron subtypes differentially regulate seizures.

Epilepsia·2026
Same author

3D pathology-guided microdissection.

Nature methods·2026
Same author

Systematic analysis of hippo pathway signaling identifies TEAD1 as a transcriptional regulator of neuroendocrine prostate cancer.

Neoplasia (New York, N.Y.)·2026
Same author

Integrative Surface Antigen Profiling of KLK2 and STEAP1 in Advanced Prostate Cancer.

Molecular cancer research : MCR·2026
Same author

Artificial Intelligence-informed Architectural Insights of 3-dimensional Glandular Networks Identify Patients With Prostate Cancer at a Higher Risk of Biochemical Recurrence.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
Same author

Tyrosine-Selective Polyfluoroaryl Modification Enables Bioactive Peptides with Enhanced Membrane Permeability and Ligation Capability.

Organic letters·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Nov 27, 2025

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
09:44

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Published on: June 13, 2018

8.4K

Multi-resolution open-top light-sheet microscopy to enable efficient 3D pathology workflows.

Lindsey A Barner1,2, Adam K Glaser1, Hongyi Huang3

  • 1Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.

Biomedical Optics Express
|December 7, 2020
PubMed
Summary
This summary is machine-generated.

A new multi-resolution open-top light-sheet microscope enables rapid 3D imaging. This innovation speeds up volumetric microscopy and diagnostic pathology workflows by allowing quick changes between imaging resolutions.

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.0K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

2.8K

Related Experiment Videos

Last Updated: Nov 27, 2025

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
09:44

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Published on: June 13, 2018

8.4K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.0K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

2.8K

Area of Science:

  • Biomedical Engineering
  • Microscopy Technology
  • Optical Imaging

Background:

  • Open-top light-sheet (OTLS) microscopy offers user-friendly, high-throughput 3D imaging for thick specimens.
  • A trade-off exists between spatial resolution and imaging/analysis time in microscopy.
  • Hierarchical multi-scale imaging workflows are valuable for volumetric microscopy.

Purpose of the Study:

  • To develop a compact, multi-resolution OTLS microscope.
  • To enable rapid transitions between different imaging resolutions within a single system.
  • To demonstrate an efficient 3D analysis workflow for diagnostic pathology.

Main Methods:

  • Development of a novel solid immersion meniscus lens (SIMlens).
  • Integration of SIMlens into a compact OTLS microscope design.
  • Implementation of a hierarchical multi-scale imaging workflow.
  • Application of the system to a diagnostic pathology workflow.

Main Results:

  • Demonstration of a compact multi-resolution OTLS microscope.
  • Successful rapid transition between air-based objectives for low- and high-resolution 3D imaging.
  • Showcasing of an efficient 3D analysis workflow for diagnostic pathology.

Conclusions:

  • The developed multi-resolution OTLS microscope facilitates efficient volumetric imaging.
  • The novel SIMlens enables rapid resolution switching, optimizing imaging workflows.
  • The system shows promise for applications in diagnostic pathology and other 3D microscopy fields.