Related Experiment Video

Updated: Nov 27, 2025

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.7K

[Effect of MiR-29b-3p Targeting STAT3 on Proliferation and Apoptosis of Acute Myeloid Leukemia Cells]

Li-Ping Lin1, Qian Zhang1, Wei Wu1

  • 1The School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, Fujian Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|December 7, 2020
PubMed
Abstract

Insights

MicroRNA-29b-3p (miR-29b-3p) inhibits acute myeloid leukemia (AML) cell proliferation and promotes apoptosis by targeting signal transducer and activator of transcription 3 (STAT3). This finding offers a potential therapeutic strategy for AML.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy.
  • Dysregulation of microRNAs and signaling pathways, such as STAT3, is implicated in AML pathogenesis.
  • Identifying novel therapeutic targets is crucial for improving AML treatment outcomes.

Purpose of the Study:

  • To investigate the regulatory role of miR-29b-3p in AML.
  • To determine if miR-29b-3p targets signal transducer and activator of transcription 3 (STAT3).
  • To elucidate the effect of miR-29b-3p on AML cell apoptosis and proliferation.

Main Methods:

  • Bioinformatic tools (TargetScan, miRanda) predicted miR-29b-3p and STAT3 interaction.
  • Dual-Luciferase reporter assay validated the targeting relationship.
  • Quantitative PCR, Western blot, flow cytometry, and MTS assay assessed gene/protein expression, apoptosis, and proliferation.

Main Results:

  • STAT3 was confirmed as a direct target of miR-29b-3p.
  • miR-29b-3p overexpression led to decreased STAT3 mRNA and protein levels.
  • AML cell proliferation was inhibited, and apoptosis was increased in the miR-29b-3p overexpression group.

Conclusions:

  • MiR-29b-3p acts as a tumor suppressor in AML.
  • Down-regulation of STAT3 by miR-29b-3p mediates its anti-leukemic effects.
  • Targeting the miR-29b-3p/STAT3 axis presents a potential therapeutic avenue for AML.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.2K
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

457

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.1K