Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-differential associations of MC3R p.F45S with human metabolic profile.

Journal of neuroendocrinology·2026
Same author

Beyond the barrier: an assessment of social behaviour in mice using a modified 3-chamber test.

Physiology & behavior·2026
Same author

The impact of self-selected light on activity rhythms in the diurnal striped mouse.

Current biology : CB·2026
Same author

Differential effects of sex and age on daily and infradian rhythms of mice.

The Journal of physiology·2026
Same author

Altered proportions of retinal cell types and distinct visual codes in rodents occupying divergent ecological niches.

Current biology : CB·2025
Same author

Editorial: Signaling pathways and brain circuitry underlying circadian rhythms and sleep.

Frontiers in neuroscience·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Nov 27, 2025

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
10:24

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Published on: May 15, 2018

15.1K

Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices.

Mino D C Belle1,2, Beatriz Baño-Otalora3, Hugh D Piggins3,4

  • 1Faculty of Biology, Medicine, and Health, AV Hill Building, University of Manchester, Manchester, UK. m.d.c.belle@exeter.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2020
PubMed
Summary
This summary is machine-generated.

This study details a method for recording neural activity in brain slices using multielectrode arrays. It provides a reliable technique for neuroscience and circadian biology research.

Keywords:
CircadianElectrophysiologyMediobasal hypothalamusMultiunit activityPerforated multielectrode arraySuprachiasmatic nuclei

More Related Videos

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
13:14

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Published on: October 26, 2014

21.0K
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
07:58

Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

Published on: August 28, 2020

9.9K

Related Experiment Videos

Last Updated: Nov 27, 2025

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
10:24

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Published on: May 15, 2018

15.1K
Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
13:14

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Published on: October 26, 2014

21.0K
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
07:58

Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

Published on: August 28, 2020

9.9K

Area of Science:

  • Neuroscience
  • Circadian Biology
  • Electrophysiology

Background:

  • Recording neural activity is crucial for understanding brain function.
  • Previous methods had limitations in scalability and consistency.

Purpose of the Study:

  • To present a detailed method for multisite extracellular action potential (AP) recordings.
  • To describe a setup for stable, high-quality recordings in hypothalamic nuclei slices.
  • To offer troubleshooting guidance for improved experimental outcomes.

Main Methods:

  • Utilizing a perforated multielectrode array system for in vitro recordings.
  • Performing recordings in suprachiasmatic and mediobasal hypothalamic nuclei brain slices.
  • Implementing a specific setup architecture for anatomical accuracy and consistency.

Main Results:

  • Demonstration of a step-by-step protocol for ensemble AP discharge frequency recording.
  • Achieved stable, high-quality recordings with excellent anatomical accuracy.
  • Provided examples of successful recordings and troubleshooting insights.

Conclusions:

  • The described method enables robust, long-term recording of neural activity.
  • This technique is valuable for advancing neuroscience and circadian rhythm research.
  • The provided guidance ensures consistency and quality in electrophysiological experiments.