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Related Concept Videos

RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Nonsense-mediated mRNA Decay02:27

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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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Detection methods of epitranscriptomic mark N6-methyladenosine.

Ye Wang1, Guifang Jia1

  • 1Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Essays in Biochemistry
|December 7, 2020
PubMed
Summary
This summary is machine-generated.

This review covers N6-methyladenosine (m6A) detection technologies, crucial for understanding RNA modification dynamics. It categorizes methods for measuring total m6A, single-gene m6A locus, and m6A sequencing to aid researchers.

Keywords:
RNAbiochemical techniques and resourcesbiotechnology

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is a key RNA modification regulating numerous physiological processes.
  • Advancements in m6A detection technologies are vital for studying its functional roles.
  • Classic antibody-based methods suffer from non-specific signals and low resolution.

Purpose of the Study:

  • To review and categorize current m6A detection technologies.
  • To provide researchers with guidance on selecting appropriate m6A detection tools.
  • To highlight the importance of accurate m6A profiling in epitranscriptomics.

Main Methods:

  • Categorization of m6A detection methods into three groups: total m6A measurement, single-gene m6A locus detection, and m6A sequencing.
  • Summary of the principles and applications of various m6A detection techniques.
  • Discussion of the advantages and limitations of different m6A detection approaches.

Main Results:

  • Identification of distinct categories of m6A detection tools based on their utility.
  • Overview of technologies enabling transcriptome-wide or gene-specific m6A site detection.
  • Emphasis on the dynamic and alternations of RNA modifications revealed by these technologies.

Conclusions:

  • The review provides a comprehensive overview of available m6A detection methods.
  • Researchers can utilize this categorization to select tools aligned with their experimental needs.
  • Accurate m6A detection is fundamental for advancing the field of epitranscriptomics.