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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: Nov 26, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Screening for functional circular RNAs using the CRISPR-Cas13 system.

Siqi Li1, Xiang Li1, Wei Xue2

  • 1State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

Nature Methods
|December 8, 2020
PubMed
Summary
This summary is machine-generated.

CRISPR-RfxCas13d effectively distinguishes circular RNAs (circRNAs) from messenger RNAs. This tool enables large-scale discovery and functional studies of circRNAs, revealing their roles in cell growth and development.

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Circular RNAs (circRNAs) are prevalent but their functions are poorly understood due to challenges in differentiating them from messenger RNAs (mRNAs).
  • Existing methods lack the specificity to distinguish circRNAs from mRNAs with overlapping exons.

Purpose of the Study:

  • To develop and validate a novel CRISPR-based tool for discriminating circRNAs from mRNAs.
  • To enable large-scale functional screening of circRNAs and identify their roles in cellular processes.

Main Methods:

  • Utilized CRISPR-RfxCas13d system with guide RNAs targeting back-splicing junction (BSJ) sites.
  • Employed a lentiviral library for high-throughput screening of human circRNAs.
  • Investigated the function of specific circRNAs, including circFAM120A and circMan1a2.

Main Results:

  • CRISPR-RfxCas13d successfully discriminated circRNAs from mRNAs.
  • Identified several circRNAs crucial for cell growth in a cell-type-specific manner.
  • Discovered that oncogenic circFAM120A promotes proliferation by inhibiting IGF2BP2 binding to FAM120A mRNA.
  • Uncovered circMan1a2's regulatory role in mouse embryo preimplantation development.

Conclusions:

  • CRISPR-RfxCas13d is a powerful tool for circRNA research, facilitating both individual and large-scale functional studies.
  • This technology advances the understanding of circRNA functions in cell growth, cancer, and embryonic development.