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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
Published on: May 22, 2018
NCBI Genome Workbench: Desktop Software for Comparative Genomics, Visualization, and GenBank Data Submission
Anatoliy Kuznetsov1, Colleen J Bollin2
1National Center for Biotechnology Information, U.S. National Library of Medicine, North Potomac, MD, USA. kuznets@ncbi.nlm.nih.gov.
Abstract:
The book chapter introduces the National Center for Biotechnology Information (NCBI) Genome Workbench, a desktop GUI software package to manipulate and visualize complex molecular biology models provided in many data formats. Genome Workbench integrates graphical views and computational tools in a single package to facilitate discoveries. In this chapter we provide a step-by-step protocol guidance on how to do comparative analysis of sequences using NCBI BLAST and multiple sequence alignment algorithms, build phylogenetic trees, and use graphical views for sequences, alignments, and trees to validate the findings. The software package can be used to prepare high-quality whole genome submissions to NCBI. The software package is user-friendly and includes validation and editing tools to fix errors as part of preparing the submission.
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