Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Function Tests01:25

Pulmonary Function Tests

566
Pulmonary Function Tests (PFTs)
Pulmonary Function Tests are crucial diagnostic tools for assessing respiratory function, particularly in patients with chronic respiratory disorders. They comprehensively evaluate lung volumes, ventilatory function, breathing mechanics, diffusion, and gas exchange. These tests help diagnose pulmonary diseases and play a significant role in monitoring disease progression, evaluating disability, and assessing response to therapy.
PFTs involve using a spirometer, a...
566
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies01:27

Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies

2.8K
Assessing and diagnosing Chronic Obstructive Pulmonary Disease (COPD) involves a detailed approach that includes a comprehensive review of medical history, physical examination, and a variety of diagnostic tests. This thorough evaluation is essential to ensure an accurate diagnosis and guide effective management strategies.
Medical History
2.8K
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

296
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
296
Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test01:22

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

84
In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess...
84
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

640
Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
640

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pulmonary hypertension associated with interstitial lung disease: a state-of-the-art review.

The European respiratory journal·2026
Same author

Screening for Pulmonary Hypertension in Interstitial Lung Disease: Preliminary Results from the PHINDER Study.

Advances in therapy·2026
Same author

Perceptions of Obesity and Weight-Related Behaviors Among Adults With Type 2 Diabetes in Benin.

Journal of nutrition education and behavior·2026
Same author

Acute respiratory distress syndrome: Why a definition matters.

Cleveland Clinic journal of medicine·2025
Same author

Acute rejection post lung transplant.

Current opinion in pulmonary medicine·2024
Same author

Oral Treprostinil is Associated with Improved Survival in FREEDOM-EV and its Open-Label Extension.

Advances in therapy·2023

Related Experiment Video

Updated: Nov 26, 2025

Author Spotlight: Integrating Alveolar-Capillary Reserve Measurements in Exercise Adaptation and Therapeutic Strategies
08:44

Author Spotlight: Integrating Alveolar-Capillary Reserve Measurements in Exercise Adaptation and Therapeutic Strategies

Published on: February 2, 2024

1.0K

Improving Screening for Alpha-1 Antitrypsin Deficiency with Direct Testing in the Pulmonary Function Testing

Samuel Gurevich1,2, Andrew Daya2, Cristiana Da Silva2

  • 1Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, Ohio, United States.

Chronic Obstructive Pulmonary Diseases (Miami, Fla.)
|December 8, 2020
PubMed
Summary

Directly testing for Alpha-1 antitrypsin deficiency (AATD) in pulmonary function testing (PFT) labs significantly increases diagnosis rates. Buccal swab testing showed the highest improvement, making early AATD detection more accessible.

Keywords:
AlphaIDAlphaKitalpha-1 antitrypsin deficiencychronic obstructive pulmonary diseasecopd

More Related Videos

Implementation of Non-invasive Point of Care Transient Elastography for Evaluation of Liver Disease in Pediatric Populations with Cystic Fibrosis
05:56

Implementation of Non-invasive Point of Care Transient Elastography for Evaluation of Liver Disease in Pediatric Populations with Cystic Fibrosis

Published on: August 29, 2025

324
Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models
03:38

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Published on: June 20, 2025

626

Related Experiment Videos

Last Updated: Nov 26, 2025

Author Spotlight: Integrating Alveolar-Capillary Reserve Measurements in Exercise Adaptation and Therapeutic Strategies
08:44

Author Spotlight: Integrating Alveolar-Capillary Reserve Measurements in Exercise Adaptation and Therapeutic Strategies

Published on: February 2, 2024

1.0K
Implementation of Non-invasive Point of Care Transient Elastography for Evaluation of Liver Disease in Pediatric Populations with Cystic Fibrosis
05:56

Implementation of Non-invasive Point of Care Transient Elastography for Evaluation of Liver Disease in Pediatric Populations with Cystic Fibrosis

Published on: August 29, 2025

324
Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models
03:38

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Published on: June 20, 2025

626

Area of Science:

  • Pulmonary Medicine
  • Clinical Diagnostics
  • Genetic Disorders

Background:

  • Alpha-1 antitrypsin deficiency (AATD) is a prevalent genetic disorder often underdiagnosed.
  • AATD can lead to severe respiratory conditions like COPD and bronchiectasis, as well as liver disease.
  • Early diagnosis of AATD is crucial for disease management and familial risk assessment.

Purpose of the Study:

  • To compare the effectiveness of different in-laboratory AATD testing methods.
  • To evaluate AATD testing completion rates initiated within a pulmonary function testing (PFT) laboratory setting.
  • To assess the resources required for direct AATD testing by respiratory therapists.

Main Methods:

  • A randomized, prospective observational study design was employed.
  • Compared direct buccal swab (AlphaID) and finger-stick (AlphaKit) testing against standard referral and prescription-based testing.
  • Testing was conducted within the PFT laboratory by respiratory therapists.

Main Results:

  • Direct buccal swab testing yielded an odds ratio (OR) of 35.14 (p<0.0001) for completion compared to standard referral.
  • Direct finger-stick testing showed an OR of 17.09 (p=0.0002) for completion.
  • Providing a prescription did not significantly improve testing rates (OR 2.61, p=0.6412).

Conclusions:

  • Direct AATD testing within PFT laboratories, particularly via buccal swab, substantially increases testing completion rates.
  • Respiratory therapists can perform AATD testing with minimal time commitment (1-5 minutes per patient).
  • Implementing direct testing strategies improves early detection of AATD and facilitates timely intervention.