Related Experiment Video
Updated: Nov 26, 2025
A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia
Published on: September 16, 2017
Characterizing spatiotemporal progression and prediction of infarct lesion volumes in experimental acute ischemia
Hai-Tao Huang1, Tao-Hsin Tung2, Min Lin3
1Department of MRI, Maoming People's Hospital, Guangdong Province, China.
Purpose:
This study was conducted to explore the diagnostic value of arterial spin labeling (ASL) combined with diffusion weighted imaging (DWI) in characterizing the spatiotemporal progression of infarct lesions in a rabbit middle cerebral artery occlusion (MCAO) model and predicting the acute cerebral infarction (ACI) volume.
Materials And Methods:
Forty-two male rabbits (2.9 ± 0.2 kg body weight) were used in this experimental study. Animals were initially anesthetized by intravenous injection of uratan. There were seven experimental groups with six rabbits in each group. The apparent diffusion coefficient (ADC) and cerebral blood flow (CBF) thresholds were established in the control group (n = 6), which were sacrificed at 12 h, stained for infarct volume, and imaged at each time point.
Results:
The normal ADC and CBF were estimated as 0.90 ± 0.03 × 10-3 mm2/s and 0.68 ± 0.06 mL g-1 min-1, respectively. The viability thresholds of ADC and CBF yielding the lesion volumes (LVs) at 3 h, which best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes at 12 h, were 0.52 ± 0.02 × 10-3 mm2/s (42.2 ± 3% reduction) and 0.33 ± 0.09 mL g-1 min-1 (51.0 ± 11% reduction), respectively. The temporal evolution of the ADC- and CBF-defined LVs showed a significant perfusion/diffusion mismatch up to 1 h (p = 0.001).
Conclusion:
ADC values and ACI volumes were positively correlated, while CBF was negatively correlated, which is supposed to be a reference for predicting ACI volume.
