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Related Experiment Video

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UBE1a Suppresses Herpes Simplex Virus-1 Replication.

Marina Ikeda1, Akihiro Ito2, Yuichi Sekine1

  • 1Department of Cell Biology, Kyoto Pharmaceutical University, Kyoto 607-8412, Japan.

Viruses
|December 9, 2020
PubMed
Summary

The Ubiquitin-activating enzyme E1 variant A (UBE1a) suppresses herpes simplex virus-1 (HSV-1) replication by reducing ICP5 protein expression. UBE1a interacts with ICP27, suggesting a role in the cellular antiviral response to HSV-1 infection.

Keywords:
E1UBA1UBE1herpes simplex virusesherpesviruslytic replicationmajor capsid proteinubiquitin activating enzymeubiquitination

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Herpes simplex virus-1 (HSV-1) causes various human diseases.
  • ICP5 is crucial for HSV-1 capsid assembly and viral replication.
  • Ubiquitination, regulated by Ub-activating enzyme E1 (UBE1), impacts viral and cellular processes, but UBE1's role in HSV-1 infection is unclear.

Purpose of the Study:

  • To investigate the role of UBE1a in HSV-1 replication and the cellular antiviral response.
  • To elucidate the mechanism by which UBE1a affects HSV-1 infection.

Main Methods:

  • Utilized UBE1a inhibitor PYR-41 and shRNA to assess HSV-1 production and ICP5 expression.
  • Performed immunofluorescence analysis to examine UBE1a and ICP5 expression levels.
  • Investigated the interaction between UBE1a and ICP27 using co-localization studies.

Main Results:

  • UBE1a suppressed HSV-1 replication; its inhibition increased viral production.
  • UBE1a expression inversely correlated with ICP5 expression, reducing ICP5 protein levels without affecting mRNA or protein degradation.
  • UBE1a interacted with ICP27 and co-localized at virus-induced chaperone-enriched domains, an interaction disrupted by PYR-41.

Conclusions:

  • UBE1a acts as a suppressor of HSV-1 replication.
  • UBE1a influences HSV-1 infection by regulating ICP5 protein expression and interacting with ICP27.
  • Findings reveal a novel mechanism of UBE1a in the cellular antiviral response against HSV-1.