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Updated: Nov 26, 2025

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
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Achieving Ultrasmall Prussian Blue Nanoparticles as High-Performance Biomedical Agents with Multifunctions.

Zhiguo Qin1, Bo Chen2, Yu Mao1

  • 1State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

ACS Applied Materials & Interfaces
|December 9, 2020
PubMed
Summary

Researchers synthesized ultrasmall Prussian blue nanoparticles (USPBNPs) below 5 nm using an ethanol/water solvent and PVP capping agent. These USPBNPs exhibit enhanced catalytic activities and potential as T1 MRI contrast agents.

Keywords:
magnetic resonance imagingnanozyme activitiesradical scavengingsolvent effectultrasmall Prussian blue nanoparticles

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Prussian blue nanoparticles (PBNPs) are vital iron-based metal-organic frameworks for biomedical applications.
  • Achieving smaller PBNP sizes for enhanced functionality has been a significant challenge.

Purpose of the Study:

  • To develop a method for synthesizing ultrasmall Prussian blue nanoparticles (USPBNPs) under 5 nm.
  • To investigate the impact of synthesis parameters on PBNP size and properties.
  • To evaluate the catalytic and MRI contrast agent potential of the synthesized USPBNPs.

Main Methods:

  • Synthesis of PBNPs using an ethanol/water mixture as solvent.
  • Utilizing polyvinyl pyrrolidone (PVP) as a surface capping agent to control particle size and prevent aggregation.
  • Adjusting the ethanol/water ratio to control nucleation and particle growth.
  • Characterization of USPBNP size, stability, catalytic activity, and longitudinal relaxation rate.

Main Results:

  • Successfully synthesized highly stable ultrasmall PBNPs (USPBNPs) of approximately 3.4 nm at an ethanol/water ratio of 3:1.
  • USPBNPs demonstrated significantly enhanced peroxidase-like and catalase-like activities compared to conventionally synthesized PBNPs.
  • USPBNPs exhibited a high longitudinal relaxation rate (r1) of 1.3 mM⁻¹S⁻¹, indicating potential as T1 MRI contrast agents.

Conclusions:

  • The developed method enables the controlled synthesis of sub-5 nm PBNPs.
  • The resulting USPBNPs possess superior catalytic properties and hold promise for T1-weighted magnetic resonance imaging (MRI) applications.