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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
17.5K
[Progress on genotyping of norovirus]
1Institute of Infectious Diseases and Endemic Diseases Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|December 10, 2020
Summary
Norovirus, a major cause of acute gastroenteritis, mutates frequently. This study reviews norovirus genotyping methods, detailing polymerase and VP1 regions for classification, aiding in understanding these evolving pathogens.
Area of Science:
- Virology
- Infectious Diseases
- Molecular Epidemiology
Background:
- Norovirus is a leading cause of acute gastroenteritis globally.
- Frequent mutation and recombination of norovirus complicate genotype identification.
- Previous genotyping focused on the VP1 region, but mutations near polymerase and capsid regions necessitate improved methods.
Purpose of the Study:
- To review norovirus sequence characteristics and genotyping principles.
- To introduce various sequence amplification methods and online genotyping tools.
- To present the latest findings on norovirus genotypes and classification.
Main Methods:
- Analysis of norovirus sequence characteristics, focusing on polymerase and VP1 regions.
- Review of established and emerging genotyping methodologies.
- Examination of sequence amplification techniques and computational tools for genotype determination.
Main Results:
- The polymerase region is classified into 10 gene groups and 76 genotypes (including tentative ones).
- The VP1 region is classified into 12 gene groups and 53 genotypes (including tentative ones).
- Tentative norovirus gene groups and genotypes require further validation and reclassification.
Conclusions:
- A two-region genotyping method, incorporating polymerase and VP1, is internationally recognized.
- Accurate norovirus genotyping is crucial for epidemiological surveillance and control.
- Ongoing research is essential to refine classification and understand norovirus diversity.
