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Updated: Nov 26, 2025

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Published on: August 6, 2016
Isolation and Purification of DNA from Complicated Biological Samples
Ruslan Kalendar1,2, Svetlana Boronnikova3, Mervi Seppänen4
1Department of Agricultural Sciences, Viikki Plant Science Centre and Helsinki Sustainability Centre, University of Helsinki, Helsinki, Finland. ruslan.kalendar@helsinki.fi.
Abstract:
The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi, and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
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