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Updated: Nov 26, 2025
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Genotyping of Plant and Animal Samples without Prior DNA Purification
Published on: September 24, 2012
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Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny
Félicien Favre1, Cyril Jourda2, Pascale Besse3
1Université de La Réunion, UMR PVBMT, St Pierre, La Réunion, France.
Methods in Molecular Biology (Clifton, N.J.)
|December 10, 2020
Summary
Genotyping-by-sequencing (GBS) simplifies genomes for high-throughput SNP discovery. This method is effective for various population and breeding studies across diverse organisms.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- Genotyping-by-sequencing (GBS) enables simultaneous genome-wide high-throughput single nucleotide polymorphism (SNP) discovery and genotyping.
- The GBS method relies on reducing genome complexity using restriction enzymes.
- Analyzing the vast amount of polymorphism data generated by GBS requires sophisticated bioinformatic approaches.
Purpose of the Study:
- To describe a simplified GBS library construction method developed by Elshire et al.
- To present recent bioinformatic tools for analyzing GBS data.
- To highlight the broad applicability of GBS in biological research.
Main Methods:
- Library construction using restriction enzymes for genome complexity reduction.
- High-throughput sequencing to generate polymorphism data.
- Bioinformatic analysis pipelines for SNP identification and genotyping.
Main Results:
- A simplified GBS library preparation protocol.
- Advanced bioinformatic methods for efficient analysis of large GBS datasets.
- Demonstrated utility of GBS for various genetic studies.
Conclusions:
- GBS is a powerful and versatile tool for genetic research.
- The described methods facilitate efficient SNP discovery and analysis.
- GBS is suitable for population genetics, taxonomy, phylogenetics, germplasm characterization, and breeding applications in diverse organisms, including those with complex genomes.
Keywords:
Genotyping-by-sequencing (GBS )High-throughput single nucleotide polymorphism (SNP )Next-generation sequencing (NGS )Plant diversity markersMore Related Videos
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