Complement receptors in human renal glomeruli. Further evidence by immunofluorescence

A new method is described for demonstrating the presence of glomerular receptors for the third component of complement in human kidney. Frozen sections are incubated together with normal human serum and inulin. Activated C3 is detected by a fluoresceinated anti-C3 antiserum. Glomeruli are labelled by C3-coated inulin particles while there is no labelling in tubules and interstitium. This method is easy and rapid and it allows the exact localization of C3 glomerular receptors.