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Negative Pressure Cell Delivery Augments Recellularization of Decellularized Lungs
Mohammadali Ahmadipour1,2, Pascal Duchesneau1, Daisuke Taniguchi1
1Latner Research Laboratories, Division of Thoracic Surgery, Toronto General Hospital, University Health Network, Toronto, Canada.
Tissue Engineering. Part C, Methods
|December 14, 2020
Summary
Negative pressure improves cell seeding in bioengineered lungs, offering a superior method for recellularization. This technique enhances cell coverage, addressing a key challenge in creating viable artificial lungs for transplantation.
Area of Science:
- Regenerative Medicine
- Biomedical Engineering
- Organ Bioengineering
Background:
- Lung transplantation is the sole cure for end-stage lung disease but faces organ scarcity.
- Bioengineered lungs via recellularization offer a promising alternative, yet face challenges with insufficient cell repopulation.
Purpose of the Study:
- To introduce and evaluate a novel negative pressure-based cell delivery method for enhanced lung recellularization.
- To compare the efficacy of negative pressure cell delivery against traditional gravity-based perfusion.
Main Methods:
- Decellularized lungs were seeded with human bronchial epithelial cells using either gravity-based perfusion or negative pressure (air removal).
- Cell surface coverage was assessed post-seeding and after 5 days of static culture using histology and image analysis.
Main Results:
- Negative pressure cell delivery resulted in significantly higher cell surface coverage compared to gravity-based perfusion.
- This enhanced cell coverage was sustained after 5 days of culture.
- Improved recellularization was also observed in lung vasculature seeded with endothelial cells using this method.
Conclusions:
- Negative pressure cell delivery is a superior method for enhancing the recellularization of decellularized lungs.
- This approach overcomes limitations in cell repopulation, advancing the potential of bioengineered lungs for transplantation.
