Evaluating the Utility of Carbon Isotope Discrimination for Wheat Breeding in the Pacific Northwest

Many wheat (Triticum aestivum L.) production regions are threatened annually by drought stress. Carbon isotope discrimination (Δ) has been identified as a potentially useful trait in breeding for improved drought tolerance in certain environments. Broad use of Δ as a selection criterion is limited, however, mainly due to an inconsistent relationship observed between grain yield and Δ and, to a lesser extent, because of the high resource demand associated with phenotyping. The efficiency of selection may be improved by the identification and verification of molecular markers for use in marker-assisted selection (MAS), and a reliable relationship to grain yield may be established based on a location's total amount and distribution of precipitation over the growing season. Given the environmental variability in precipitation dynamics, it is necessary to evaluate this relationship in target breeding environments. In this study, grain Δ was collected on a panel of 480 advanced soft white winter wheat varieties grown in five Pacific Northwest environments. A genome-wide association study approach was used to evaluate the amenability of grain Δ to MAS. The genetic architecture of grain Δ was determined to be characterized by multiple, small effect marker-trait associations with limited repeatability across environments, suggesting that MAS will be ineffective at improving Δ selection efficiency. Further, the relationship between grain yield and Δ ranged from neutral (r = -0.01) to moderately positive (r = 0.44) in the target environments. Such moderate correlations, coupled with variability in this relationship, indicate that direct selection for Δ may not be beneficial.