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Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
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Concordance testing between Powerplex ESI 16 Fast System and VeriFiler Express.

Hagit Schayek1, Ortal Waiskopf2, Ksenia Rashkovski1

  • 1DNA Database Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, Jerusalem, Israel.

Journal of Forensic Sciences
|December 14, 2020
PubMed
Summary
This summary is machine-generated.

Different DNA STR kits can produce varying results for the same genetic loci, impacting database accuracy. Laboratories must account for these inconsistencies when using new STR kits like VeriFiler™ Express to ensure reliable DNA profile analysis.

Keywords:
DNA databasePowerplex ESI FAST kitVeriFiler Express kitallele dropoutdiscordance

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Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Short tandem repeat (STR) kits are crucial for DNA profiling in forensic science.
  • Variations in allele calls between different STR kits can hinder data sharing and database comparisons.
  • Expanding analyzed loci is necessary for handling degraded samples, low DNA quantities, and complex mixtures.

Purpose of the Study:

  • To compare genotyping data between the VeriFiler™ Express and Powerplex™ ESI FAST STR kits.
  • To quantify the discordance rates and types of mismatches observed between the two kits.
  • To highlight the importance of addressing inter-kit variability in DNA analysis.

Main Methods:

  • Genotyping of 1568 DNA samples using both VeriFiler™ Express and Powerplex™ ESI FAST kits.
  • Comparative analysis of allele data generated by the two STR kits.
  • Identification and categorization of discordant genotyping results.

Main Results:

  • A discordance rate of 1.27% was observed between the VeriFiler™ Express and Powerplex™ ESI FAST kits.
  • Allele dropout mismatch occurred in 0.89% of samples, and additional fixed-size third alleles in 0.38%.
  • Observed discordance rates exceeded previously reported rates for the VeriFiler™ Express kit.

Conclusions:

  • Significant inter-kit variability exists in STR genotyping, necessitating careful validation.
  • DNA laboratories and genetic-match search systems must consider these inconsistencies for accurate profile interpretation.
  • Standardization or robust cross-validation protocols are essential for reliable DNA database management and casework.