Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid ligand binding and membrane interactions of a novel food-derived lipid transfer protein enhance basophil allergic responses.

Scientific reports·2026
Same author

Controlled human malaria infection in adults identify combinations of merozoite antigens associated with clinical immunity.

Nature communications·2026
Same author

The impact of cytomegalovirus coinfection on tuberculosis in the mouse model.

BMC infectious diseases·2026
Same author

B cells and iBALT in TB immunity & pathogenesis.

Frontiers in immunology·2026
Same author

Species-specific iNOS expression distinguishes epithelial and myeloid IFNγ responses in tuberculosis.

bioRxiv : the preprint server for biology·2026
Same author

Sex Differences in Vaccine-Induced Immunity and Protection Against Mycobacterium tuberculosis.

The Journal of infectious diseases·2025

Related Experiment Video

Updated: Nov 25, 2025

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
12:48

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

Published on: January 2, 2013

13.0K

BCG Provides Short-Term Protection from Experimental Cerebral Malaria in Mice.

Julia Witschkowski1, Jochen Behrends2, Roland Frank3

  • 1Junior Research Group Coinfection, Priority Area Infections, Research Center Borstel-Leibniz Lung Center, 23845 Borstel, Germany.

Vaccines
|December 15, 2020
PubMed
Summary

The tuberculosis vaccine BCG prolonged survival in mice with experimental cerebral malaria (ECM). BCG reduced brain inflammation and immune cell infiltration, but not parasite levels, suggesting a novel protective mechanism against severe malaria.

Keywords:
BCGPlasmodium berghei ANKAexperimental cerebral malariaheterologous immunitynon-specific protection

More Related Videos

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
09:04

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging

Published on: June 8, 2017

6.7K
Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
08:37

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

Published on: November 18, 2010

17.8K

Related Experiment Videos

Last Updated: Nov 25, 2025

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
12:48

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

Published on: January 2, 2013

13.0K
In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
09:04

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging

Published on: June 8, 2017

6.7K
Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
08:37

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

Published on: November 18, 2010

17.8K

Area of Science:

  • Immunology
  • Infectious Diseases
  • Vaccinology

Background:

  • Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection, causing significant mortality.
  • The Bacillus Calmette-Guérin (BCG) vaccine, primarily for tuberculosis, shows cross-protection against other pathogens.
  • BCG's protective effects against malaria, particularly cerebral malaria, require further mechanistic investigation.

Purpose of the Study:

  • To evaluate the efficacy of BCG vaccination against Plasmodium berghei ANKA (PbA)-induced experimental cerebral malaria (ECM) in C57BL/6 mice.
  • To elucidate the immunological mechanisms underlying BCG-mediated protection in experimental cerebral malaria.

Main Methods:

  • C57BL/6 mice were immunized with BCG prior to infection with PbA.
  • Survival rates and clinical symptoms of ECM were monitored.
  • Parasite burden, pro-inflammatory mediators, and CD8+ T cell infiltration in the brain were assessed.

Main Results:

  • BCG vaccination significantly prolonged survival and reduced ECM development in PbA-infected mice.
  • BCG-mediated protection waned over time and was associated with viable BCG in organs.
  • Protection was not linked to reduced parasite burden but to decreased pro-inflammatory mediators and CD8+ T cells in the brain.

Conclusions:

  • BCG vaccination confers partial protection against experimental cerebral malaria by modulating brain inflammation and immune cell infiltration.
  • The findings suggest BCG's protective effect is independent of direct anti-parasite activity.
  • Understanding these mechanisms could inform the development of adjunctive therapies for human cerebral malaria.