Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber.

Area of Science:

• Biomedical Engineering
• Materials Science
• Analytical Chemistry

Background:

• Hemoglobin (Hb) level determination is crucial for diagnosing blood diseases.
• Graphene oxide (GO) offers excellent optical properties and biocompatibility for biochemical detection.

Purpose of the Study:

• To develop an ultrasensitive hemoglobin sensor using a graphene oxide-coated microfiber.
• To leverage GO's properties for enhanced Hb molecule binding and detection.

Main Methods:

• Coating an optical microfiber with graphene oxide to create a biosensing platform.
• Utilizing evanescent light field enhancement and abundant bonding sites for Hb.
• Real-time monitoring of dynamic bonding via optical spectrum analysis and surface morphology characterization (SEM, Raman).

Main Results:

• Achieved ultrahigh sensitivity of 6.02 nm/(mg/mL) and a detection limit of 0.17 μg/mL.
• Demonstrated 1-2 orders of magnitude lower detection limit compared to existing fiber optic Hb sensors.
• Confirmed successful GO deposition and dynamic Hb bonding through morphological and spectral analysis.

Conclusions:

• The GO-coated microfiber sensor provides a highly sensitive, low-cost, and fast method for Hb detection.
• This technology presents a competitive alternative for blood disease diagnosis and warrants further medical research.